1. Here we show that a feedback loop connects the levels
of sigma K
and SpoIIID, such that production of sigma K leads
to a decrease in
the level of spoIIID.
2. Induction of the Bacillus subtilis kinA gene, which
codes for a
major kinase of the phosphorelay pathway does not required
the genes
spo0A, spo0B, and spo0F at the onset of sporulation.
3. Induction of the Bacillus subtilis kinA gene, which
codes for a
major kinase of the phosphorelay pathway , required the
spo0H gene,
coding for the sigma H protein, but not the genes spo0A, spo0B, and
spo0F at the onset of sporulation.
3. Induction of the Bacillus subtilis kinA gene, which codes for a
major kinase of the phosphorelay pathway, required the spo0H
gene,
coding for the sigma H protein, but not
the genes spo0A, spo0B, and
spo0F at the onset of sporulation.
4. The existence of the feedback loop was demonstrated by using
antibodies prepared against SpoIIID to measure the level of spoIIID
during sporulation of wild-type cells, mutants defective in sigma K
production, and a mutant engineered to produce
sigma K earlier than normal.
4. The existence of the feedback loop was demonstrated by using
antibodies prepared against SpoIIID to measure the level of spoIIID
during sporulation of wild-type cells, mutants
defective in sigma K
production, and a mutant engineered to produce sigma K earlier than
normal.
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5. The sigma H factor, on the other hand, is dispensable for the
switch in the position of the ftsZ assembly site.
6. Previous studies showed that SpoIIID
is needed to produce sigma K,
but suggested that spoIIID represses sigma K-directed transcription of
genes encoding spore coat proteins.
6. Previous studies showed that SpoIIID is needed to
produce sigma K,
but suggested that spoIIID represses sigma K -directed transcription of
genes encoding spore coat proteins.
7. Induction of the Bacillus subtilis kinA gene , which
codes for a
major kinase of the phosphorelay pathway , required the
spo0H gene,
coding for the sigma H protein
7. Induction of the Bacillus subtilis kinA gene , which codes for a
major kinase of the phosphorelay pathway , required the spo0H
gene,
coding for the sigma H protein
7. Induction of the Bacillus subtilis kinA gene , which
codes for a
major kinase of the phosphorelay pathway , required
the po0H gene,
coding for the sigma H protein